Hi everyone,
I am now following a silver staining protocol which will add 20ul formaldehyde together with 0.125g silver nitrate in 50ml water. However, according to my knowledge formaldehyde will reduce silver ion into metallic silver. Therefore, I am curious that will the sensitivity decrease due to some of the silver ion reduced into metallic silver and fail to bind to protein?
PS. This protocol work quite well so i'm just curious if this ''problem'' is really a problem.
Thank you!
Ellen Chu
Protein staining with silver begins with the binding of positive silver ions to negative protein ions. The remaining free silver ions are removed by washing the resulting gel. Then silver ions on the protein are reduced to metallic silver with an ammonia solution of formaldehyde. The problem is the good laundering of free silver ions. Wash off until a precipitate of silver iodide is added to the filtrate with the addition of potassium iodide.
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