What is the protocol for the digestion restriction enzyme of phage?

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Michael J. Benedik

If you have clean phage DNA then the protocol for digesting it is no different than for a plasmid. But aware that a few phages have modified DNA that doesn't digest (T4 is a good example) although most are fine.

What do you mean by what markers?

Amy Klocko

Do you mean which DNA marker (as in ladder)? The marker/ladder is chosen to help interpret band sizes. Phage genomes are small, and you're planning to digest it. So, perhaps 100 bp DNA ladder?