My team wants to study the cell wall architecture of Vigna radiata roots by using SEM. Is there a clear protocol for preparing root sections for SEM Analysis?
To processing vigna samples for SEM microscopy, you can use pattern protocol, fixing the samples (I think that you use little samples of Vigna, around two or three milimeter) in glutaraldehyde or paraformaldehyde 2,5% in 0.1 M in sodium phosphate buffer. Then, you can dehydrated the sample in an ethanolic series until 100% followed by critical point drying with CO2, how is explained in this link (http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46667/751375148952).