I am researching the Folliculinids in Australia and will be using SEM, which I have not used before. Do I need to narcotize them before fixing and what is the fixation protocol?
I know nothing about folliculinids, but may be this paper can be of use for you:
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0485.2006.00118.x/full
It looks like the protocol they used in paper is a standard protocol for SEM. A lot of them are on the Web, for example:
http://www.cmif.osu.edu/Methods-SEM
Hi Vlad, many thanks for the leads, they are just what I needed.
Regards John.
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