What is the protocol for fixation, whole mounts, and paraffin embedding for histological cut in polyclads?

How can I measure MMP-1 protein activity or protein level from liver tissue?

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How to compare two groups with only two measurements?

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

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Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

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What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

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Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

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Do we need to write protocol for a second study of a systematic review that has been registered and published?

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How to increase a model accuracy ?

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Chlorinated organic formation in odor control scrubbers using hypochlorite?

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Saleh Alkarim

Hello

Look at links here .

Good Luck

http://www.protocolsonline.com/histology/sample-preparation/paraffin-processing-of-tissue/

https://scholarspace.manoa.hawaii.edu/bitstream/handle/10125/1154/v28n4-361-373.pdf?sequence=1

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300233

https://books.google.com.sa/books?id=64jgZ1CfmB8C&pg=PA37&lpg=PA37&dq=(+histological+study+of+polyclads+tissue+)&source=bl&ots=q7uRz6qyqK&sig=3azzqP7Y3iGdgKAWA6HOJw5gadw&hl=ar&sa=X&ved=0ahUKEwiFkMuTxfnRAhVIwxQKHeqoDPMQ6AEINTAD#v=onepage&q=(%20histological%20study%20of%20polyclads%20tissue%20)&f=false

Rita Smirnov

The a very important point is fixation.You may use fixative Zenker's or Carnoy's or Boiuin's (less good).Alcohol processing is very long: each stage takes 6 hours(60%-70%-80% -90%-absolute-3 times absolute +csilol- csilol/And paraffin 3 times.

Katiria Soto Diaz

For fixate your specimens you can use Boiuin's, for me works really well but before start with the washes you have to clean your specimens with PBS. I like to use fast washes with alcohol (70%, 80%, 85%, 90%, 95% and 2 times with 100% EtOH for 15 minutes each one) and methyl salicylate (2 times per 15 mins each one) before use paraffin or glutaraldehyde, I prefer paraffin, because is more user friendly.