Kindly provide information about literature from when I can get the protocol.
Mr. Banerjee ... There is a standard protocol for quantification of Beta-Casein, but there is no quantification method for A2 Beta-Casein. It is one type of genetic variant, it cannot be quantified, There are test methods which can qualitatively estimate for presence or absence for A2 Beta-Casein.
Dear Banerjee,for detection of beta casein A2 in milk samples, PCR-RFLP or Allele specific PCR can be done.
A single amino acid in the milk protein beta-casein makes the difference between A1 and A2. The A2 casein at position 67 contains the amino acid proline, and the A1 casein histidine. Thus, in the digestion of A1, the so-called opiate beta casomorphin-7 (BCM-7) is split off, not in the A2 varinates. This metabolite is said to be the reason that some people can not tolerate milk.
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