What is the protein control to check for mitochondrial purity from nuclear/cytosolic contaminants?

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Christian Q. Scheckhuber

Hello Ravi,

for detecting cytosolic contamination: anti-alpha-tubulin,

for detecting nuclear contamination: anti-calnexin

Mitochondrial control: anti-VDAC

Best regards!

Moêz Smiri

the marker enzymes: fumarase and catalase activities for mitochondrial and cytosol fractions, respectively.