Flush with organic solvent: Start by flushing the column with an appropriate organic solvent, such as methanol or acetonitrile, to remove any residual carbomer sample. This helps to solubilize and remove the carbomer from the column.
Rinse with water: Follow the organic solvent flush with a rinse using distilled water or an aqueous solvent to further clean the column. This helps to remove any remaining organic solvent and impurities.
Backflushing: In some cases, backflushing the column may be recommended. This involves reversing the flow direction of the mobile phase through the column to dislodge and remove any remaining particles or contaminants.