How can i detect the phenol red in mixture solution?

Hi. I'm trying to detect the phenol red in the mixture solution by HPLC. As you know, It is using for ph indicator. The problem is.. After making phenol red solution in 50 ml conical tube, I...

13 December 2020 5,511 4 View

How to prevent protein gelation?

Hi :D I have a question. We expressed a protein from E. coli and we got purified peptide. But when we concentrate it with centrifuge filtration (like amicon), It became like a gel. So when we...

05 October 2020 3,727 2 View

Bioanalyzer: Additional Peak in 28s Region?

Hi All, I isolated RNA from human cell line using TRIzol reagent and sent isolated RNA for RNA-seq analysis. The company sent me a QC report showing that some of my samples has additional peak at...

13 September 2020 1,334 3 View

How can solve the problem during Gromac calculation?

Hi :D I'm using Gromacs 5.1.5 for MD simulation. For binding energy calculation, I used g_mmpbsa (version 5.1.2) I simulated the protein for 150ns and nojump of my xtc file. Then cut last 50ns for...

25 August 2020 7,589 1 View

How can i purify my protein sample?

Hi :D I'm doing purify a small protein from E. coli. From E. coli extract supernatant, I purified my protein using IMAC purification with LC. I got a single peak at chromatogram and got the...

23 July 2020 2,393 5 View

DNA fragment digestion without a restriction enzyme site?

Can I digest a specific DNA sequence without a known restriction enzyme site in vitro? I am trying to digest a fragment with CGTCAT but not CATCAT. I tried cas9 with a sgRNA containing the target...

20 July 2020 9,320 4 View

How to determine the number of cells before suspending them in 1x binding buffer in Annexin V FITC assay?

I am performing Annexin V FITC assay for adherent cells. According to the protocol described, the treated cells were trypsinised and collected, followed by washing them twice with cold PBS. The...

18 July 2020 8,695 5 View

How can I prevent protein fragmentation in solution?

Hi, all :D I'm doing short protein expression (~17 kDa, pI 9.6) from Bl21de3 with c-term His tag. I purify it by IMAC (or affintiy chromatography). When I do SDS-PAGE and WB with Anti-His Ab, I...

25 May 2020 6,693 6 View

How to deal with chance constraints in Matlab toolbox ga?

I am learning chance constraints. I want to know how to deal with chance constraints with generic algorithm, especially in matlab toolbox ga? Thank you a lot !

15 March 2020 3,472 3 View

Peptide adsorption to filter membrane? or denatured?

Hi:) I'm doing His tagged peptide expression and purification from E.coli. I expressed the peptide and checked it with WB (with Anti-His Ab). For purification, I did IMAC and got an eluate. But it...

12 March 2020 6,399 13 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Is it possible to set a breakpoint at a specific DYNAMIC instruction using the GNU Debugger (GDB)?

Using GDB, it is straightforward to debug and monitor a target program by setting a break-point at a specific instruction, since the instruction addresses are known. However, we have two...

24 February 2021 1,598 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

Why CHi^2 is reducing if I divide the intensity by a constant factor?

I was refining my XRD data in FullProf. Highest intensity was 400000. The fitting was good but still the Chi^2 value is very high (0.174E+04) I divided the intensity by some constant factor (say...

17 February 2021 3,383 5 View

Simulation or Experiment? How do we choose?

If we want to assess if a phenomenon is going in a direction we expect, we can simulate it, using digital models, possibly agent models factoring in known properties and interaction features. We...

12 February 2021 1,141 21 View