Yes there is bacterial growth, but you had so many cells on the plate to begin with that is grew into a lawn. It is hard to tell what you were attempting to do, but if this was a transformation then you did not have a functional selection. Either your plate did not have the antibiotic or the strain was already resistant. If you were doing something else, then you need to describe it. But the plate started with millions or more of cells.
Yes. This is a bacterial growth on agar plate. But It is not clear what is your goal. have you did an antibacterial test?
Sharareh Harirchi I have put the antibacterial strips at the broth, then I culture it in agar plate
Michael J. Benedik I think I had a mistake when I was culturing the bacteria, I have shaked the broth with bacteria before I taked a sample from it to growth on agar plate, is this actually my problem??
Hi again. You have to do antibacterial test based on standard protocol.
Islam Al-Shurman It is impossible to give you an answer without knowing what you did, it would be helpful if you provided more details. What is the experiment you are doing, what is the bacterium, what is the medium you are using, antibiotics? How long did you shake the broth? What dilution?
Michael J. Benedik I have a polymer had antibcterial effect, I fabricate it as tablet then I put them in a M.H broth with E.coli after 24 hours after that I shaked the broth then I took a sample by swab and cultivate on M.H broth
I think you have a problem with your experimental design. After 24 hours you are almost certainly going to have a saturated culture, even with an antibacterial polymer some cells will become resistant and grow out to final density. Also just swabbing from the culture is not going to let you count. You need to incubate cells with your polymer for a few hours then prepare serial dilutions and count the surviving cells to get an actual number and compare to some appropriate set of controls. Or else you need to monitor the optical density of the culture over a time course to show that a culture with the polymer table is growing much slower than the control.
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