What is the Principle of Discipline?

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Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

Hello.. please how can I prepare an emulsion with nile red and nile blue dyes for visualization under fluorescent microscope ?

how can I incorporate the dyes to each phase ? do I have to heat them !!

21 June 2024 9,377 1 View

Is the Journal of Pedagogical Research is a trusted journal to publish in? I check in scopus and it is there but I have concerns if it is predator?

Is the Journal of Pedagogical Research a trusted journal to publish in? I checked in scopus website and scimago and I find it is there with scopus Q2 rank; but I have concerns if it is predator?...

08 June 2024 7,678 2 View

Topics for my PhD in TOPSIS?

I'm considering research topics for my PhD in TOPSIS methodology. Could you suggest some potential titles or areas of study that would be both relevant and impactful?

26 April 2024 602 2 View

Seeking Advice: K-Points and Band Configuration for Phonon Calculations?

I'm currently working on phonon calculations using Phonopy and VASP DFPT approach with a 2x2x1 supercell. I'm seeking guidance on: K-Points Selection: How should I determine the appropriate...

29 February 2024 3,955 0 View

Why may I get a Ct value that is higher for one of my treated samples than my control ( zero hour in a time course study) in RT qPCR??

I'm conducting a time course study on gene expression using RT-q PCR for samples treated with 4 conditions: vehicle, RA agonist, Calcitriol agonist or a combination of both agonists over 6 hours....

26 January 2024 7,921 1 View

How to dilute essential oil before antioxidant assay?

What are the solutes used to dilute essential oil for DPPH, TPC, FRAP and Beta/Carotene Bleaching Assay? Can I simply dilute the essential oil using 1 mg/mL in methanol/dH2O for all assay?...

21 November 2023 2,599 10 View

What style or the behavior of battery with draw compare with solar cell?

with draw the behavior of battery that meaning IV characteristic

09 November 2023 8,991 1 View

What is the possible reason why no emulsion formation in beta carotene/linoleic acid bleaching assay?

The procedure that I used 2 mg beta carotene in 10 mL chloroform with 200 mg Tween 40 and 20 mg linoleic acid. Next, chloroform removal by rotary evaporator and 50 mL of distilled water is added.

08 November 2023 1,890 2 View

What is the standard for Beta carotene/linoleic acid bleaching assay?

What is the standard/positive control that can be used instead of Butylated hydroxytoluene (BHT) or Butylated hudroanisole (BHA)? Sample: Plants’ essential oils

06 November 2023 2,250 8 View

What can i do to find the right topic for my thesis?

i am facing the difficulty to find the right and latest topic for my thesis. i searched and read hundreds of articles but i couldn't find the right one. what can i do I'm really confused.

02 August 2024 1,323 8 View

Do we save MSMEs of the Nation ?

SMEs are backbone of the country's white economy, most of them are II generation entrepreneurs and migrated from family owned business policies, and having sufficient higher educations for...

01 August 2024 5,562 2 View

How to choose the journal?

Hello I want a suitable journal in the field of remote sensing and machine learning to be judged quickly. Thank you for your guidance Thanks

01 August 2024 1,799 4 View

In teaching with Evidence-Based Practices for students with ASD, what are some that can benefit from the use of AI?

I am a student in a master's program for Special Education and I am curious to see what Evidence-Based Practices that are used in teaching individuals with Autism Spectrum Disorder benefit from...

30 July 2024 5,611 1 View

If the emission of all greenhouse gases is stopped now, will the temperature of the earth continue to rise?

Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...

30 July 2024 5,123 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

I am looking for the permission to use the OLBI survey. Does anyone know where I can obtain that information?

I have reached out to the original creator Dr. Demerouti but have had no luck with a response. I am completing my IRB form and need the information or permission by tomorrow. I know short notice,...

29 July 2024 3,684 0 View

In terms of chaos, what is the necessary and sufficient condition for authoritarianism, permanent or temporary, to come to exist and persist?

Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...

28 July 2024 6,515 1 View

What is effective targeted chaos?

Perfect democracy thinking assumes no chaos so no need for independent rule of law system and liberal democracies assume the possibility of normal democratic chaos that can be sorted out by an...

28 July 2024 473 1 View

AutoGrid and Autodock 4.2.6 window opens for 1 second then closes and there is no output files?

Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...

28 July 2024 2,136 4 View