What is the Power-Train-Efficiency of Diesel engine locomotive in %?

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Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

What is the difference between Q sepharose column and DEAE Sepharose column?

I am working on protein (lipase enzyme) purification. After using the Sephadex G-25 fine column, which column (Q or DEAE sepharose column) should I use? What are the buffers should I select? Thank you

16 February 2021 6,921 5 View

How to use ArcGIS to separate certain % of data points scattered over certain area?

I was able to make a grid using fishnet in ArcGIS. But after that, I need to select 20% of points from each cell and make a different file containing ids of the data points as testing data set....

07 January 2021 9,533 1 View

How to quantify the two components overlapping in HPLC?

I am analyzing the biodiesel using reverse-phase HPLC. Methyl Oleate and Methyl Palmitate are overlapping in a single peak. How can I quantify the individual components from the same peak?

14 November 2019 1,406 9 View

I am working on a medical imaging research and if anyone knows where I could find CT scans of the brain with and without contrast agents?

I have looked up Cancer Imaging Archive but I need a bigger dataset. Can anyone help me out with some good places to look at for more images with and without contrast agents?

28 October 2019 4,219 3 View

Formula to calculate the plating cell volume based on the doubling time, culturing time, desired confluency and the volume of the culture flask?

Hi I am working on various cancer cell lines now. For my experiments, doubling time is really very important. Because even a small percentage of dead cells will affect my measurements. I have...

09 June 2019 6,592 6 View

Difference in carbon footprint between imported and locally produced food?

Field to fork studies....any papers or on going research?

28 May 2019 5,334 3 View

How to troubleshoot the IF staining problems in skin tissues?

Hi We are working in the area of psoriatic diseases. We are everytime getting the problem in expression of protein in dermis. We unable to see the expression in epidermis. Even, we did IHC too...

29 November 2018 4,074 1 View

Why is in situ hybridisation preferred over immunohistochemistry in paraffin embedded mouse embryo sections?

I am trying to characterize early embryos and most literature I have read seems to prefer in situ hybridization over immunohistochemistry to study expression profiles of different proteins. Does...

13 November 2018 8,911 1 View

What are the biological assays for drug and TNF Alfa binding studies?

Dear sir/madam, Please suggest the biological assays which need to prove the drug and TNF Alfa binding studies. Thanks.

07 June 2018 8,093 1 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

Is it mandatory to specify Subjects Combinations in Generalized Linear Mixed Models (SPSS)?

Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...

24 February 2021 7,379 4 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View