I feel confused about the term pH at the point of zero charge, does it mean the value of pH of the solution before apply electrical potential on it (i.e. before polarize it)?
The isoelectric point is the value of pH at which the colloidal particle remains stationary in an electrical field. In systems in which H+/OH− are the interface potential-determining ions, the point of zero charge is given in terms of pH.
It is the pH at which the net charge on the particle is zero or the particle possess equal number of positive and negative charges. Once this pH is reached, particles stop moving in the presence of electric field.
Is that mean it is founded when we apply potential to the electrolyte and after completing the ion-exchange between the electrolyte and the membrane surface?
And what about the conditions in EGFET pH sensors in double-layer region:
1- If pH>pHpzc then the result will be negative ions (-).
2- If pH=pHpzc then the result will be (H), which means neutral.
The pH of an electrophoretic gel is determined by the buffer used for that gel. If the pH of the buffer is above the pI of the protein being run, the protein will migrate to the positive pole (negative charge is attracted to a positive pole). If the pH of the buffer is below the pI of the protein being run, the protein will migrate to the negative pole of the gel (positive charge is attracted to the negative pole). If the protein is run with a buffer pH that is equal to the pI, it will not migrate at all. This is also true for individual amino acids. Wicipedia
pH is the negative logarithm of the activity of hydrogen ions.
The charge of one electron 1,602176487(40)×10−19 Кл =1 (+1or -1) or 0
For my work, instead of protein I used CuS thin film to be an EGFET pH sensor, and used the buffer solutions from (2-12) step 2. That's mean I start from a high acid up to high base. And the reference electrode acts as the positive pole, while the CuS sensing film acts as the negative pole. And I have the pH of the CuS membrane (let's say 9), so when I apply acid (low pH, which means high [H+]), it will be be below pH of the sensing film, then the hydrogen ion (positive charge) will migrate to the negative pole which is the sensing film itself, and vice versa.
What do you mean by PI, is it the (Point of Isoelectric)?
Really it is fantastic explanation, many thanks to you Dear Dr. Yuri Mirgorod
I did not receive your question. On the result of my work, the management of RG impose sanctions to reduce my rating. Sometimes he does not show me my scientific topics. I should look to the planting of PI CuS as well as you.