What is (the origin of) the horizontal components of ground reaction force?

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My question is about errors in Ansys mechanical APDL? Data file file.db does not exist for RESUME?

I did a welding simulation using ANSYS MECHANICAL APDL. The solution was done, but I encountered this error in the output file (Data file file.db does not exist for RESUME). On the other hand, I...

11 July 2024 4,480 0 View

What Remote Sensing Software Is User-Friendly for Urban Ecology Research?

Hello everyone, Our lab is currently looking to purchase user-friendly remote sensing software, particularly for individuals without a background in remote sensing or coding. Ideally, we are...

05 June 2024 2,940 6 View

By which acid can we replace sulfamic acid to eliminate nitrite in a soil extract to determine the nitrate content by the DEVARDA method?

by which acid can we replace sulfamic acid to eliminate nitrite in a soil extract to determine the nitrate content by the DEVARDA method

26 May 2024 5,822 1 View

Could you provide me with the ISO protocol for measuring nitrites and nitrates in soil?

Could you provide me with the ISO protocol for measuring nitrites and nitrates in soil?"

25 May 2024 252 0 View

Nitrate dosage KCl?

Can I apply the nitrate dosage method in water to the soil extract with KCl?

25 May 2024 8,169 4 View

How we can add PIN Diode in CST ??

Hello All i want to design a reconfigurable antenna for which i have to use PIN diode as a switch. anyone help me how to add PIN diode in CST i mean in schematic ?? Thank you

04 March 2024 8,098 1 View

I have a new article and I want to publish it in your journal , how can I do this?

I have a new article and I want to publish it in your journal , how can I do this? I have defended my dissertation recently and taken a short article from it

14 February 2024 673 3 View

CO and Cross polarization using HFSS or cst?

Greetings, Please how can i plot Cross or CO polarization on both hfss and cst Thank you

15 October 2023 4,393 2 View

Immunofluorescent marker for SH-SY5Y cells?

Hi everyone. we are doing IF for checking the translocation of a protein between cytoplasm and nucleus in SY5 cells , and I need a marker better than NeuN to visualize the whole cell and make it...

07 August 2023 9,942 4 View

Protocol for an acid protease test?

Hi everyone What is the protocol for an acid protease test, excluding the use of hemoglobin? I am seeking cost-effective testing protocols. #acid protease #protocol

29 July 2023 4,145 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,404 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 840 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

What is the right voltage sweep direction for characterization of HER kinetics?

In general, when performing a Tafel analysis for corrosion resistance measurements, a line voltage scan is performed from say -250 to +250 mV vs. OCV at low scan speed around 0.125 mV/s. However,...

25 July 2024 7,982 3 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View