When growing purified human primary colorectal carcinoma cells in NOD SCID mouse subcutaneous xenografts, tumor-associated stroma and vasculature populating the human tumor tissue are of mouse origin. I'm now trying to derive 2D-cultured cell lines from those xenografts (propagated several generations in mice). In some cases, there is outgrowth of tumor-associated fibroblasts.
I wonder of which mouse tissue origin are those fibroblasts? Are they mouse skin-derived?
Has anybody experience with fibroblast eliminating agents such as FibrOUT (Chi-Scientific)?
Those kits are tissue specific, so which one should I choose in this setting?
Thanks in advance for your help!
Auto-answering... after one or two passagesof human xenograft tumors in mouse the stroma and vasculature seems to be mainly of mouse origin.
Hylander et al. Journal of Translational Medicine 2013, 11:110
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