Hi everybody,
I would like to exploring if manipulation of a specific gene of interest in female mice PFC alters dendritic branching/morphology. I have never done this before and was wondering what the best slice thickness would be to look at this. Would 200um sections be optimal? I would like to visualize my staining using a confocal microscope.
Thank you in advance!
Changzheng Zhang
90 micrometers is enough for most neurons, such as cerebellar Purkinje cells, cortical pyramidal neurons.
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