I want to extract fungi and bacteria DNA from skin swab. Some articles used two 5mm steel beads per sample, some used 0.4-0.6mm ceramic beads per 0.5g sample. And other diameter for beads were also mentioned in different articles. It is said that bigger diameter has stronger ability to destroy fungi cell wall. However, I am afraid that the bigger beads would hurt DNA of bacteria.
Which diameter is safe and suitable for DNA extraction from both fungi and bacteria.
Thank you very much!