I am trying to set-up LTD experiments on acute rat brain slices in vitro.
I tried to prepare slices from P12-P14 and P18-P21 Wistar male rats and used low-frequency stimulation (1 Hz, 15 min) for LTD induction.
Both in the GD and in the CA1 the depression of fEPSPs was very weak and un-stable.
I would greatly appreciate an advice about the best age of rats and a good induction protocol.
Hi, i have experience with 7 week old rats for LTD experiments (2-3 month old mice too) and used used a strong LTD induction protocol. for more details check
sajikumar et al., 2007
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Binu, thank you for your answer!
I checked the paper by sajikumar et al., 2007 you cited, but I've only found there LTP, but not LTD induction protocols. Could you, please, share your protocol for LTD?
Okay then please check
Sajikumar and Frey, 2003, 2004
Synaptic tagging and cross-tagging: the role of protein kinase Mzeta in maintaining long-term potentiation but not long-term depression.
Sajikumar S1, Navakkode S, Sacktor TC, Frey JU.
Best
can look up the work of Cliff Abraham (Wickliffe C Abraham) at University of Otago in Dunedin, NZ. Specifically look at all his early to mid-90s work on LTP/LTD leading to ideas on metaplasticity. Much of the work was done in slices, although some done in vivo as well.
Can find on Research Gate.
https://www.researchgate.net/profile/Wickliffe_Abraham
Sorry, but my question was about LTD, not LTP. A admit, however, that astrocytes may be also important for depression modulation. Do you know anything about?
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