I am working with alginate and chitosan immobilization of bacterial cells. I am experiencing some difficulties in the assessment of the process yield, in what concerns to determining the viable number of cells after spray drying. I am now resuspending the spray dried product in NaCl 0,85% and make successive dilutions for spreading. However, after 24h, few cells have been released.
1. Alginate should be soluble even in water (without NaCl). But in this case NaCl is necessary for bacterial cell osmotic stability.
2. Chitosan is soluble in diluted acids (HCl or acetic acid).
3. Do you see any visual changes in your suspension upon addition to NaCl solution? Are particles completely disintegrated. If not, try with magnetic stirrer to break particles.
4. Are you used proven protocol for spray drying of bacterial cells? Conditions during spray drying are critical for cells viability.
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