Dear researchers, I need to know your insight/opinion on the most suitable lysis buffer to be used on mouse preimplantation embryo for preparation prior qPCR. In my case, I just need to preserve/isolate RNA only. In addition, how long can the embryo be keep after adding lysis buffer until it will be used for qPCR? Thank you in advance.
Your question is not clear for one cell of the embryo or for the whole embryo, or on which days of the embryo. Also, it is clear of what RNA will be isolated. If for mRNA, the mRNA should be protected well not be broken by the RNAase.
Ke-Hui Cui Thank you for your response. I am sorry for the confusion. To clear my question, it is for the whole embryo at 2- and 8-cell stage.
Ke-Hui Cui Thank you for your insight. I will look forward into it.
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