What is the most reliable mitotic marker for adult human brain slices?

01 January 2018 1 3K Report

Using immunofluorescence, I tried Ki67 and anti-pH3 on paraffin sections but the stainings have a high background and an unexpected high number of cells in the subiculum light up.

Elliot Glotfelty

You may find some useful info here:

https://www.researchgate.net/post/What_is_the_best_method_for_antigen_retrivial_for_ki67_staining_on_frozen_sections

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