I would like to have an idea of which cell-based assays have been used in different laboratories to assess antioxidant activity.
The antioxidant potential in vivo (yeasts cells) - the critical concentration, which is defined as the cross between the viability and mortality lines
doi:10.3390/pharmaceutics11040191
Immunoblotting with SOD and CAT will give you the intracellular antioxidant status.
I'd like to recommend cellular antioxidant activity (CAA) assay. https://doi.org/10.1021/jf0715166. It had been established in different labs all over the world.
https://www.researchgate.net/post/What_is_the_best_method_to_use_to_measure_antioxidant_activity_in_a_small_volume_of_isolated_exosomes_from_cancer_cells
To measure oxidative stress and the ability to oxidize in the plant is preferred to use
As for the animal, it is preferred to use clotathione
Assays like Superoxide dismutase (SOD), Catalase, Gluthionine perioxidase, Ascorbic acid etc. By using this, antioxiant acitivity can be measured.
I would like to give my opinion on calorimetric method. This method is very promising. Please have a look on:
Article Simultaneous Kinetic and Thermodynamic-Based Assay to Determ...
Article Erratum to: Food and ascorbic scavengers of hydrogen peroxid...
I think almost everyone in the field could be interested in this research article.
Article Should we ban total phenolics and antioxidant screening meth...
I recommend to read the papers and reviews of Professor Pincemail (University of Liège, Belgium). We should not expect one universal biomarker of redox status or antioxidant activity. Live cells may activate various detoxification or repair systems according to the organ they come from and the type of oxidative stress applied.
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