I would like to stain the spines of adult born granule neurons for the purpose of density and morphology analysis. Does anyone have a positive experience with a particular fluorescent staining?
I have only performed such analysis with the aid of retroviral targeting of adult born neurons. Now I would like to simply stain the spines, without resorting to viral delivery. As I doubt DCX on its own would be enough for staining spines, I was thinking of using DCX and Acting together. Does anyone have experiences with such (or any other) double staining?
Thank you in advance for your information and time.