What is the most efficient way to quantify the level of gluten in wheat flour?
the response in french:
here is a current method to determine gluten!
Teneur en gluten : NA-730-1990
Le gluten est la fraction des protéines insoluble dans une solution saline ; il est le responsable majeur de la qualité rhéologique des pâtes. Le dosage de gluten repose sur son insolubilité dans l’eau chargé de sels. Extraction est réalisée par la méthode manuelle
Principe :
Préparation d’une pâte au moyen d’un échantillon de semoule ou farine et d’une solution Na Cl (2.5%).
L’isolement du gluten humide par lixiviation de cette pâte avec la solution de chlorure de sodium, puis essorage et pesage du produit obtenu
Expressions des résultats
Le gluten humide et le gluten sec sont exprimés en pourcentage :
*Gluten humide g :
La teneur en gluten humide % : GH= M1 x 10
M1 : masse du gluten (g).
*Gluten sec :
La teneur en gluten sec % : GS =M2 x 10
M2 : masse de gluten sec (g)
*Capacité d’hydratation : CH%= (GH-GS) X 100
from Prof Amir y. Ummto Algeria
in the french typed method the amount of flour to use is 10 g
herein is another method:
Wet and dry gluten
Wet and dry gluten contents of flour were estimated by following the method No.
38-10 as described in AACC, 2000. 25 g of flour was taken in a porcelain cup. Sufficient
water was added to form firm dough. Dough was hand kneaded into ball and placed it in
water at room temperature for 60 min. Dough was kneaded gently in stream of tap water
over bolting cloth until starch and soluble matter were removed. To determine whether
gluten was starch free, one or two drops of wash water, obtained by squeezing, were
dropped into clear water. The gluten thus obtained was pressed as dry as possible
between the hands, and weighed as wet gluten. Then it was transferred to an air oven to a
constant weight at 100 °C for 24 hours and cooled. It was weighed as dry gluten.
prof AMIR Youcef ummto, Algeria
https://www.celiac.com/articles.html/what039s-the-best-way-to-measure-gluten-in-gluten-free-foods-r4240/
Article Gluten Detection Methods and Their Critical Role in Assuring...
- Badges
- Science topic
- Similar topics
- Correction