Since you talked about infected RBCs, I assume you are interested in calculating parasite density of malaria parasites. I agree with you that the calculation of parasitemia needs to be standardized as the formulae used to calculate it are dependent on calculating the number of parasites against a certain count of either RBCs or WBCs. For example, an assumed number of WBCs (8,000/ul) is usually set as a criterion. However, this could be convenient in certain settings or geographic areas but not in others. Another problem is that counting could be done using inappropriate sections of the blood smear. Therefore, over-estimation or under-estimation could result when calculating parasite density, particularly if performed by under-trained microscopists. In my opinion, each endemic area should have a pre-determined average count of WBCs to be set as the assumed count for future calculations (if WBC counts cannot be done alongside with parasite counts for each patient). It would be fine to hear from experts in the field and we are waiting to hear from them.

Kind regards,

Rashad

I would agree that counting WBCs would be the best method. However, in the past I have had to count RBCs. I have attached the paper where I counted RBCs; please see the "Microscopy" section for both the method and the formula that are standard.

Article Co-infection and cross-species transmission of divergent Hep...

Thank you Prof Rashad for you answer.

Thank you Dr Ghai for your kind answer, have a good luck for ur PhD

The traditional method is to count the number of infected rbcs against 200 wbcs

You may find this article usful: http://www.malaria journal.com/content/12/1/428; Malaria Journal 2013, 12:428. It contain: Estimating the parasitaemia of Plasmodium falciparum: experience from a national EQA scheme

If you need to estimate parasitaemias due to trypanosome infections you need to do this using a haemacytometer in a WBC diluting fluid

Hi

I quite find this an opportunity to ask something about parasite count. from my database, there were various in reporting the parasite count, for example % parasitemia, in relative with white cell count and plus system. May i know which of these methods measure the gametocyte count? is it in relative with white cell count of which an assumed number of WBCs (8,000/ul) is the criterion?

https://www.researchgate.net/publication/236252192_Co-infection_and_cross-species_transmission_of_divergent_Hepatocystis_lineages_in_a_wild_African_primate_community

Article Co-infection and cross-species transmission of divergent Hep...

https://www.researchgate.net/publication/236252192_Co-infection_and_cross-species_transmission_of_divergent_Hepatocystis_lineages_in_a_wild_African_primate_community

Article Co-infection and cross-species transmission of divergent Hep...

 For Percentage parasitemia you can calculate by this method 

Number of infected RBCs / Total RBCs *100 

Or 

By Flocytometry you can use any DNA staining dye which can .. be detected .. at particular wavelength... there are many paper on flowcytometry... But the Microscopy is the Gold Standard till date ...and it is cost effective no need to spend more money ... 

hello

https://www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC)

thank you

Kindly follow the attached file.

Is the calculation of infected  RBC X 100 over the total number of RBC which is 1000 then you will know the % parasitemia

Infected RBCs /Total RBCs x 100 percentage Parasitemia

Percentage Parasitemia = 100 x Infected RBCs /Total RBCs

The percentage parasitaemia should be calculated by counting the number of parasitised red cells in 1000 cells in a thin blood film.

I think its okay with the analysis of clone of parasite in blood/ days, and monitor the temperature chart in patient's parasitemia.