The transmission electron microscopy (TEM) images, revealing the interior structure of the yeast cell (Saccharomyces cerevisiae), are shown below. The cells contain many vacuoles (V) and electron-dense aggregates probably formed from the virus-like particles (VLP) as a result of the transient transfection (Figure A, B, D). On Figure C, the filament (F) formation was observed near the nucleus (N) presumably due to the presence of the virus or metabolic dysregulation (Petrovska et al., 2014).
All critical comments regarding my TEM images are highly appreciated.
Interesting that there are extremely few mitochondria and peroxisomes in the pMD9-containing yeast cells. Furthermore, the cell membrane and the nuclear membrane look highly irregular, but this could also be due to the fixation. How do your control cells (i. e. not containing pMD9) look like?
Interestingly, we did not observe the electron-dense aggregates in the yeast cells (control) not transfected with the pMD9 plasmid.
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