What is the morphology of the RAW 264.7 cells that polarizes to M1 macrophages by LPS+IFN g stimulation?

More Jhuwala Venkatakrishnan's questions See All

How to measure fluorescence intensity of Dapi stained nuclei?

I have tried various methods using imageJ to measure mean fluorescence intensity of dapi stained nuclei but none of them seem to be working. Ive attached an image i am trying to measure the...

15 February 2021 8,952 3 View

Anyone have suggestions for a good High School Sophomore project in Computational Biology using NMR?

The scope of research is 6-8 months. Student attending regular school should be able to demonstrate her knowledge of the field and outcome should be such that the student can be an author of the...

04 September 2019 6,802 0 View

How can i measure the capacitance of a metal oxide apart form calculation from CV ?

06 January 2016 1,030 3 View

What is the relationship between hardness and strength of an alloy? Especially magnesium alloys?

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06 May 2015 4,646 13 View

What is the potential window for CV studies of CuO modified electrodes?

I am going to study the ORR , by CuO modified GC electrodes. Different papers give different potential range for studies.pl. clarify

02 March 2015 4,246 4 View

How can I calculate mass gain in TGA tests?

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27 January 2015 7,999 2 View

What are the various factors causing variation in the formant frequencies in speech?

What are your consideration while detecting speech? For example- sex(m/f), Age , BMI, emotion , pitch etc

09 September 2013 351 1 View

Which controller is used for designing a MAV?

Any type of MAV.

22 May 2013 8,814 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

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Why activated CAR-Jurkat cell could not kill targets?

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

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04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

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04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

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How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

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01 August 2024 476 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

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How to culture and maintain Lx-2 cells?

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Shen-An Hwang

There is no morphological differences between a M1 and M2. You may be able to use certain surface markers, but that is also getting a bit muddied. Best is a combination of surface markers with cytokin production.

And to keep in mind... m1 and m2 are fluid, meaning they can switch between the two depending on the environment.

Johannes Westman

M1 are in the shape of a "fried egg" while M2 are elongated.

See (Article Contrasting phagosome pH regulation and maturation in human ...

)

Jhuwala Venkatakrishnan

Thanks Shen-An Hwang!

Thanks Johannes Westman !