Hi all,
I have found loads of paper to fix rat testes but plenty of protocol variations.
I have started to fix some testes in NBF 10% for 24h but it doesn't look enough fixed.
Do someone perforate the tunica for better action of fixative? Or maybe fix it for 48 hours?
I am open to protocol modification but also precise that I won't change the use of NBF.
Kind regards
Stephane
We use a 26g needle to poke 10 holes at both ends just enough to puncture the tunica but not deep enough to cause damage to the tubules.
And make sure you have provided enough fixative to more than cover the tissue. Leave at RT for the first 24 hours, then move to the refrigerator for another 24 or more hours before trying to cut them.
We've switched to using Davidson's fixative for the first 24 hours followed by NBF.
- Badges
- Science topic