CPD(cyclobutane pyrimidine dimer) id DNA damage product during UV exposure.Many research have been conducted by ELISA or immunostaining to check CPD expression but I am not able to find any research done by western blotting. Anyone have any information regarding its molecular weight in kD ?, please share.
Hi!
Read this paper;
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454621/pdf/nihms566706.pdf
regards
I would recommend you read this paper:
http://www.jbc.org/content/279/31/32950.full
They reported that the NMR analysis of the DNA recognition mechanism by T. thermophilus CPD photolyase had a molecular weight of 48 kDa.
Hi Daniel.Thanks You for your valuable suggestion but photolyases are DNA repair enzymes that repair damage caused by exposure to ultraviolet light and I am searching for CPD molecular weight(cyclobutane pyrimidine dimer), a DNA damage product.
Hi Juan, I read that article where cpd is detected by RIA and other methods. My concern is to know its protein mol.wt in kD as I am doing western blotting but i am unable to find that.
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