Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How to Implement Elastoplasticity in a PCL model in COMSOL Multiphysics?

I am using COMSOL Multiphysics 5.5. I am trying to simulate the tensile test of PCL scaffolds. However, regardless of the strain, I have the elastic region all the time which is not true when it...

23 February 2021 4,050 1 View

Can you help me with a free /no cost publication journal in oncology Nursing?

Wish to publish an article but in a cost-free journal. Need the assistance of researchers on good indexed journals free of predatory. Thank you

23 September 2020 5,372 3 View

How do you differentiate your different populations in your FASTA file in R ?

I need to identify my populations from my FASTA file in R so that R can then run all my stats. However, I do not know how to tell R how to group my data into 4 populations! This is the warning...

09 September 2020 8,875 2 View

Can anyone please post a link to a reliable rat cDNA primer database?

I am looking for a source of cDNA primers, rat genome, real time PCR. Previously I used rtprimers bd, but is seems to be out of service for a while now.

01 September 2020 5,132 3 View

How to generat a ASCII format data in GIS?

Dear you all: Recently i met with such a problem: i tryed to generate a ascii format buffer in gis, but i failed because it's headers are different from the research data. Failed result are shown...

11 August 2020 5,040 3 View

Do 3 light sources with same intensities, but different wavelengths(blue,green, red) provide same amount of energy for photosynthesis?

As it is not directly my field, I would like to ask for help in terms of the correlation between light intensity, wavelength, frequency and amplitude. Let's say, I have a radiophotometer which...

29 April 2020 9,141 2 View

Constitutive expression of a gene in E. coli - In search of plasmid?

Dear All, I am currently working on a project based around an antibiotic resistance enzyme and I got some really interesting results. I am looking to confirm whether the enzyme under...

19 June 2019 7,700 4 View

How does one identify and extract statoliths from cubozoans?

Specifically, I am looking at the species Alatina alata. I am having trouble both finding the statoliths and also extracting them for further analysis. I have been freezing specimen before I am...

24 April 2019 7,943 2 View

Which test to use in SPSS?

So i have a study with both males and females, they will both be shown a negative image and their brain reaction recorded and a positive image with their brain reaction recorded what analysis in...

30 March 2019 4,571 6 View

No title

03 March 2021 5,360 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

Could anyone recommend papers that focus on mixed-ethnicity Black-Caribbean/Caucasian adopted children?

Dear All, I have been looking for papers, or books, that focus on the issues surrounding adoption of mixed Black-Caribbean/Caucasian children. I am interested in literature that looks at any...

22 February 2021 8,466 2 View

Any way to separate out fluorescent channels from a composite image?

Hi there, I've been using the EVOS Auto II microscope for some time now and usually I will save the transmitted light/bright field image and the images from fluorescent channels as separate...

15 February 2021 546 3 View

Please, How can I plot a channel capacity of MIMO and SISO in matlab?

CSISO= f(frequency) : plot(f, CSISO) ? CMIMO= f(frequency) : plot (f, CMIMO) ?

13 February 2021 5,225 4 View

What can I do to keep magnetic beads suspended in solution within a syringe?

I'm looking for ways to prevent the settling of magnetic beads in solution. I have been using a syringe pump to inject a suspension of magnetic beads in PBS into a microfluidic device, and so far...

09 February 2021 6,206 2 View

What/How to focus on with regard to research publication?

I'm an academician for past 10 years and love to continue with the same, a question pops-up at in mind from time to time, what to focus more on? Conference papers, Journal paper, book chapters,...

09 February 2021 1,009 5 View

The reason of PMMA channels cracking?

07 February 2021 3,663 2 View

How can I reduce run-to-run variation in TMT MS experiments?

I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...

31 January 2021 3,405 3 View