In plant cryo-banks, the number of samples processed per accession varies widely. What is the minimum standard of post-cryopreservation viability that you are working on your program? How many shoot tips are processed per accession to reach it?
This great paper on seed preservation is from CGIAR genebank. The CGIAR collections include >700,000 seed accessions. Please find the information below:
https://www.sciencedirect.com/science/article/pii/S2211912421000663?via%3Dihub
CGIAR genebank viability data reveal inconsistencies in seed collection management
The number of samples processed per accession varies widely and is dependent upon technical staff availability, material availability, desired security level and purpose of storage. There are no specific rules on the number of replicates that should be held in storage. This quantity is intrinsically related to the security level desired, which in turn it is linked to the purpose of the storage samples.
Reference: https://link.springer.com/article/10.1007/s11240-020-01846-x
- Badges
- Science topic
- Similar topics
- Geoscience
- Asia
- India