Before buying the reagents specific for particular germ-line, I want to get a clue about the differentiation potential of my stem cells (iPSCs) in vitro. For that, what are the minimum essential components (medium + reagents) needed? Is it essential to use specialized differentiation medium at the beginning or just DMEM will work? Can we use High glucose or low glucose DMEM or Knockout DMEM is required?
Please answer.
Your questions are very broad. There are 200 papers published on iPSCs and ESCs in Pubmed. I will suggest that read carefully you will find all the answers in them. Reagents for iPSCs and differentiation are expensive. Based on your purpose you designed media carefully.
Subhash C. Juneja Thank you for your suggestion. Actually I read many of them but their conditions vary from each other which makes me confused. So, I am looking for an expert opinion If I could get.
Thanks, Nasir, let us see if someone comes up the right plan for you, I myself was indirectly involved in PSCs work for making cartilage, I was only involved in animal surgeries part in this paper (pl see enclosed a pre-print). On the other hand, I am expert in MSCs area.
- Badges
- Science topic
- Similar topics
- Social Psychology
- Attitude