What is the minimum amount of sample to start an RNA sequencing experiment (for both human and mice blood samples)?

More Rana Mumtaz Raoof's questions See All

How to do pca analysis of c-alpha atom of the protein?

i m interested in pca analysis of c-alpha atoms in gromacs for that i used the following gmx_mpi covar -s mdca.tpr -f mdca.xtc -o eigenvalca.xvg -v eigenvecca.trr -av average.pdb -n index.ndx but...

30 July 2024 1,607 1 View

Getting this error : gmx_mpi': corrupted size vs. prev_size: 0x0000000001d1b320 ***?

i am using the trjconv command in gromacs gmx_mpi -debug trjconv -s md.gro -f md.xtc -o utr.xtc -fit rot+trans and after few steps i got the error gmx_mpi': corrupted size vs. prev_size:...

17 July 2024 6,216 1 View

How i figuer out the weight of curcumin powder?

I am trying to load curcumin emulsion in 4% chitosan gel but i don not know how many mg powder is needed ?

10 July 2024 2,462 1 View

Any suggestions to enhance the wettability and adhesion of fluorosilanes?

I am facing a difficulty to prepare the thin film which contains fluorine contained silica monomer, as we know F is highly reactive with low surface energy and it could be the reason of low...

09 July 2024 6,017 3 View

Why is the mouse intestinal organoid not forming ?

I am isolating the crypt from mouse small intestine the following several modification of EDTA concentration, 3mM (30 min), 5mM (30 min), 20mM (25 min) at 4 degree. For all the EDTA...

09 May 2024 9,005 2 View

What correction is used when 95%aCI is overlap ?

How we make 95% CI (lower and upper) far, when overlap in analysis, whether we can use effect size to make difference in lower and upper interval

03 May 2024 1,846 2 View

Is Pluriselect a good company to buy kits for cell separation?

I am recently buying kits from Pluriselect to take out (deplete) NK cells and CD4 cells from blood. The main reason me using this is cheap and do not require accessory equipment. But in Pubmed...

02 May 2024 5,861 1 View

WARNING: FENE bond too long: 1 7 8 32968539.141547877 (src/MOLECULE/bond_fene.cpp:83) ERROR on proc 0: Bad FENE bond (src/MOLECULE/bond_fene.cpp:85)?

# Polymer chain simulation with FENE and LJ potential in NVT ensemble # Initialization units lj # Use LJ units atom_style bond # Atoms and bonds boundary p p...

16 April 2024 5,828 0 View

Bond broken in fene potential?

i simulating a single chain polymer with 10 particle with fene and lj but if i increase the no of particle my bonds are being broken . im using parameters as below 20 50000.0 0.1 1000.0 1.0...

15 April 2024 2,748 0 View

How to calculate the center of mass coordinate of the protein in gromacs?

i want to find center of mass coordinate of the protein,for that i using the following command gmx_mpi traj -f md_noPBC.xtc -s md.tpr -com -ox com.xvg -pbc but i m not getting satisfactory...

04 March 2024 814 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View