Any papers related to the topic of extraction of optical properties of tissue-mimicking phantoms?

Dear Community, I'm trying to find some information about the extraction of optical properties of phantom without the usage of spectrophotometer. Thanks in advance

21 December 2020 6,770 3 View

How to interpret Generalised Linear Model output from SPSS?

I am trying to run an ordinal logistic regression using Generalised Linear Model from SPSS. There variables are A,B,C,D, and a moderation variable A*C. Variables A and D are continuous variables...

02 December 2020 5,159 3 View

What is the Timestep definition in the Conservation Element / Solution Element in 3D ?

15 November 2020 1,214 3 View

How to isolate Clostridium from mixed culture?

My Clostridium tyrobutyricum seems not producing spores. I incubated the Clostridium tyrobutyricum in 10 ml of RCM at 28 degrees Celcius for 5 days anaerobically, then heat the whole tube in 75...

25 October 2020 3,386 1 View

What's typical DNA yield and quality of DNeasy Powered Food Kit?

I spiked in 4-8 log CFU/ml of Lactococcus lactis into milk, and use DNeasy Powered Food Kit to extract DNA from it. Nanodrop 2000 was used to determine the values. The yield and quality are weird:...

04 October 2020 6,211 4 View

Flow Cytometry help?

Dear All, With Flow cytometry, people would buy a specific antibody and it's associated isotype control. What i would like to ask is, if my antibody is mouse IgG2b, could i use the an isotype...

05 May 2020 9,595 4 View

Is free cytosolic GFP fluorescence excluded or reduced in the endomembrane system (ER, Golgi, lysosomes)? Why or why not?

As soluble, untagged GFP is not membrane bound and doesn't contain targeting or signal sequences, one might expect it to be synthesized by free ribosomes and not bound ribosomes. That would mean...

08 March 2020 997 3 View

How many base pairs mismatches should i consider to design a specific primer? my templates have 1-2base pairs mismatch only when i aligned them?

i need to design a specific primer for each of my samples. but my samples, when i aligned them, they only have 1-2 bp mismatches, barely 3

17 September 2019 6,650 3 View

Can you recommend a fluorescent ELISA agent?

Using a fluorophore rather than a chromophore for ELISA detection seems like an obvious solution to increasing ELISA sensitivity, but I'm struggling to find work done in this area. Can anyone...

07 August 2019 2,604 5 View

What are the Arch Garch conditions?

20 April 2019 3,704 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can I get the design of circular cylinder to fix thermocouple on the surface and cartridge heater in core ?

Respected Scientific Community. I'm interested to carry out experimental studies on a circular cylinder. But even after doing literature review I unable to get the exact design of the cylinder...

10 February 2021 6,221 1 View

Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,090 3 View

What molecules bind to dsDNA?

Hello, there are some molecules that bind to double-stranded DNA; for instance, SYBR green and some antibodies. Are there more examples? Thank you

25 January 2021 7,595 4 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View

How is linear form of plasmid circularized?

Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...

13 January 2021 8,336 4 View

How can I calculate packing density of particles by fomulas or softwares?

I have all data of particles, such as size distribution, or numbers. Is there any way that I can calculate the packing density of these particles? Assume that all particles are circular.

05 January 2021 4,272 2 View

Low value of Cd for Flow Past a Square Cylinder Simulation?

Hi everyone, Happy New Year! I am carrying out a Simulation for Flow Past a Square Cylinder. I am using Ansys Fluent. I get a very low value of Cd in the simulation. The solution converges but...

03 January 2021 4,389 3 View

How to add two vertical and parallel Fresnel reflection coefficients to retrieve overall reflection coefficient for a right hand circular wave?

I have a problem in which a right hand circular polarized wave (GPS wave) encounters an ideal smooth plane obliquely, and then is reflected off that. To solve this kind of problems, the wave is...

28 December 2020 2,271 3 View