As most of the serum-gel separator tubes end-up in the biological hazardous waste bin after serum has been taken for serology test, I am currently experimenting the usefulness of the clotted blood from candidaemia samples (result known by serology test) that may contain Candida sp. cells and its DNA could be extracted and applied as a template in PCR. Please advice other methods than Steven et al. 2007 (attached) as this method to obtain human DNA, and not yeast DNA, which the cells are lesser and seems to stuck with gel separator and fibrin during fragmentation process.
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