What is the meaning of big black dots (which seems to be DAPI negative) in a DAPI stained nucleus of a C2C12 cell?

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For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How can we identify (in silico) the interacting amino acid residues or the nucleotides involved in the Protein-Protein / Protein-RNA interaction?

Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...

14 July 2024 950 2 View

Any suitable Animal models to evaluate Mast cell stabilizers?

I am looking for an in-vivo model to evaluate standardized herbal extract for its potential as a mast cell stabilizer.

14 July 2024 779 0 View

What is the mathematical expressionof theoretical yield strength calculated considering solution strengthening in stainless steel 316L?

In the expression for solid solution strengthening model that has been widely used in the literature use a linear form consisting of strength coefficient, and concentration of elements in wt.%....

11 July 2024 4,476 3 View

How to insert cholesterol inside DPPC membrane by replacing same number of DPPC molecules in GROMACS?

I am trying to insert cholesterol inside the DPPC membrane by using gmx insert_molecules -replace. But number of DPPC molecules replaced is much higher than the number of cholesterol inserted?...

30 June 2024 4,160 2 View

How to synthesize Dichloro(p-cymene)ruthenium(II) dimer in a good yield?

Suggest some procedure where i can synthesize [Ru(p-cymene)Cl2]2 in a good yield.

23 June 2024 9,244 2 View

Analysis of microbial biomass for nutrient cycling?

It will be an honor, if anyone can suggest the standard methodology for analyzing nutrients(N,P,K) in soil microbial biomass

23 June 2024 3,850 4 View

Can i use polystyrene as a matrix in a hybrid COMPOSITE?

fiber powders will be used as a reinforcement.

23 June 2024 10,027 3 View

After expert validation of the research tool for data collection, what should be done first after piloting. Reliability analysis or factor analysis?

Which would be prefered first (factor analysis or reliability analysis of a Likert type scale)

19 June 2024 9,378 4 View

Which media will produce more biomass in saccharomyces cerevisiae?

Dear all, For higher biomass for saccharomyces cerevisiae which media is suitable in shake flask? 1. YPD media 2. Basal salt media and what is the optimum C/N ratio ( 5:1 /10:1). Thanks &...

18 June 2024 2,106 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

Expansion thermal of muscle ?

What is the thermal expansion coefficient of human muscle?

27 July 2024 7,190 0 View

For anyone that works with Human Aortic Smooth Muscle Cells(HASMCs), what seeding density do you use when plating?

I am struggling to find a seeding density that will allow my cells to not aggregate together. Anything helps!

24 July 2024 3,460 1 View

Does anyone have sources where I can find the contraction ratios (% shortening of muscle length during full contraction) of specific muscles?

I founde some textbooks stating that typical skeletal muscles can shorten up to 70% (optimally up to 30%) during contraction. However, I would like to look at muscles per region. This might seem...

21 July 2024 399 3 View

Does EMG show muscle spasms?

Electromyography waveforms reflect the electrical activity of a muscle. But does it reveal muscle spasms (transient) or cramps (prolonged spasm)? If so, what does it look like?

20 July 2024 5,951 1 View

Are my cells contaminated with mycoplasma?

I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...

11 July 2024 7,786 3 View

Flow cytometry compensation with beads - is the matrix the problem?

I am trying to compensate my flow cytometry data. I have human tumor tissue, dissociated and stained with live/dead fixable::APC, EpCAM::FITC, CD45::PE. I also stained and measured single stain...

07 July 2024 5,614 5 View

Why does my Modified Gomori Trichrome (LG) Stain muscle fibers red, and how can I improve it?

I am trying to visualize Nemaline Rods in Skeletal Muscle fiber cross sections. I have read about 10 different gomori protocols, and the protocol seems very straightforward. Every paper I read on...

27 June 2024 5,423 2 View

Have you had problems with Vectashield Vibrance with DAPI?

The DAPI stain turns yellow after 24+ hrs and I loose most Alexa 488/594 signal. Product has not expired, standard double label immuno protocol I have used for 20+ years. Had this problem in the...

24 June 2024 397 1 View