What is the meaning of big black dots (which seems to be DAPI negative) in a DAPI stained nucleus of a C2C12 cell?

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How can I find the effect of different parameters like (Reynolds number, Hartman number, volume friction etc.) in COMSOL?

Here I am facing this problem. I don't find any effect of this type of parameters. I am a beginner user of COMSOL.

23 February 2021 9,086 3 View

Can i quantify protein concentration in sds page?

I have to quantify a mixture of proteins. But I can not quantify its concentration by any protein detection methods as the values fluctuate. Can i quantify the protein concentration by sds page?

22 February 2021 215 2 View

Can fractal dimension of thin film surfaces affects the electrons energy states?

As fractal dimension of a thin film surface implies the irregularity in the surface. Is it possible that the energy states of the electrons are also affected by the fractal dimension?

19 February 2021 5,509 3 View

Can we use image data in MATLAB Neuro Fuzzy Designer ?

I have remotely sensed data (image) that I want to use in the Neuro-Fuzzy Designer apps of MATLAB. Is it possible to use an image file in the Neuro-Fuzzy Designer app or only numeric data is...

17 February 2021 1,205 5 View

Is it possible to determine % K2O of organic manures by STPB method ?

To determine % K2O of organic manures, flame photometric technique is used whereas in inrganic fertiliser for K2O determination STPB method is used. I am trying to find out any method of...

24 January 2021 4,714 3 View

How to tackle "CONSTRAINT CONTROLS" error while using tie between CFRP and frame segment in abaqus?

Hello, I had been trying to model a portal frame wrapped with CFRP in ABAQUS. To tie the CFRP laminates with frame I need to use slave/master surface interaction. But whenever I try to model the...

19 January 2021 2,906 3 View

How to remove colour from DNA pellet during DNA extraction?

I am working with Amaranth accessions which are green to red in color and contain high phenolic compounds. Using the CTAB method with the slight modification I am trying to get high-quality DNA so...

18 January 2021 3,578 1 View

Why the mixture of NaOH solution (10-12M) and Na2SIO3 solution is getting solidify after mixing?

These days I am noticing that the mixture of NaOH (NH) (10-12M) and N2SiO3 (NS) solution gets solidify after 24 hours. The ratio of mixture of NS to NH is 2. To mention, the same solutions that we...

18 January 2021 7,911 2 View

Which model is most suitable for urban simulation?

Respected sir/mam, I want to simulate the urban expansion using different time series LULC based on satellite image. please suggest me most suitable model for urban simulation. Thanks and regards.

17 January 2021 9,134 11 View

Is there any packages or code of MATLAB for solving a system of stochastic delay differential equation?

Hello, Can you help me regarding MATLAB solver for a system of stochastic delay differential equations? To be specific the system is delay differential equation where parametric stochasticity is...

15 January 2021 6,950 7 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View

How would I use a glycerol fixation to fix whole brain Drosophila tissue?

I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.

21 February 2021 3,081 3 View

Could someone help me troubleshoot my NOS assay protocol for skeletal muscle extracts, please?

I am trying to measure differences in eNOS activity in skeletal muscle in sedentary and exercised mice, following the conversion of 3H-arg to 3H-cit, and assaying the samples with and with out...

31 January 2021 8,324 1 View

What is the best way to minimize/prevent the effects of rigor mortis in excised mouse muscle?

I'm planning to excise mouse muscle to use for ex vivo mechanical testing. What is the best way to prevent the stiffening effects of rigor mortis from confounding results if I cannot isolate the...

30 January 2021 1,641 2 View

Need for help. What goes wrong with my differentiatio process of C2C12?

Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...

30 January 2021 9,535 2 View

Non motile black dots in animal cell culture, tightly adhered to the cells along the cell periphery. Is it a contamination? How to get rid of them?

I am seeing non-motile black dots of almost equal size along the periphery of skin melanoma cell line A375. They are present in almost all the cells. They don't turn the media turbid. However...

18 January 2021 5,151 1 View

Co-stain Lectin PNA FITC conjugate with an uncojugated primary antibody?

Hello, I am planning to stain retinal tissue sections for IHC and want to co-stain cones and rods. For this we plan on using a Lectin PNA From Arachis hypogaea (peanut), FITC Conjugate for cones...

12 January 2021 583 1 View

Simultaneous imaging of multiple components (Carbohydrates, Fat, Protein) in food products using confocal microscopy??

Hi Everyone, I am trying to characterize the microstructure (localization of carbohydrates, fats, and proteins) of coconut milk powder using confocal microscopy. From the review of the available...

12 January 2021 5,464 2 View

Fluorojade-C troubleshooting experience?

Hello all, I've tried a Fluorojade-C stain according to the manufacturer's recommendations on FFPE tissue sections. My problem is that there is no staining, not even the DAPI control. I prepared...

11 January 2021 9,749 0 View