How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How is the quality of archived blood sample for HbA1c test?

HbA1c is an important indicator of long-term glycemic control with the ability to reflect the cumulative glycemic history of the preceding two to three months. Also, HbA1c is measured as the ratio...

16 September 2020 2,423 4 View

Which primer pair is better for amplifying endophytic fungal communities in roots of trees while avoiding host amplification?

Which primer pair is better for amplifying endophytic fungal communities (metabarcoding with Illumina sequencing) in roots of trees while avoiding host amplification? I would greatly appreciate a...

03 September 2020 728 3 View

Bacterial siderophore production test/modified CAS assay results interpretation?

I've been working with plant bacteria and investigating their ability to produce siderophores. For a qualitative test I chose a method described hereModified microplate method for rapid and...

26 July 2020 7,194 4 View

What is the methodology for the isolation of Bacteria endophyts?

ISOLATION OF ENDOPHYTIC BACTERIA Hello! Please what are the next steps after obtaining several colonies after seeding the Nutrient Agar medium by the mass seeding technique? In short, I have a...

06 April 2020 6,112 9 View

Does SIM (Sulfide Indole Motility) medium work for obligate aerobes?

I work with obligate aerobic bacteria and need to check if they are motile. I was thinking of using SIM medium, but it occurred to me that it might not work with obligate aerobes. Can anyone clarify?

25 March 2020 9,555 3 View

Any mathematical study for sickle cell disease?

Any mathematical study for sickle cell disease?

21 February 2020 4,857 4 View

For how long can I keep leaf samples at 4 degrees Celsius before I isolate endophytic microorganisms ?

I intend to do Isolation of bacterial and fungal endophytic microorganisms from leaves collected from several sites. Is it possible to collect the leaf samples, store them at 4 degrees Celsius and...

04 February 2020 5,705 4 View

What could be a gel like, viscous, material in a endophytic bacterial culture (in liquid suspension culture)?

We have isolated a bacterial endophyte that grows well in MS media and afetr some 20 days in culture without subculturing, we witnessed some clear, gel like,very viscous granules sticking on to...

11 December 2019 7,155 3 View

Which biochemical modification of Hemoglobin can potentially carry more oxygen to bloodstream?

Any synthetic analogue of Hemoglobin or any other Xenobiological agents can be cited as example. Please provide links and references.

20 November 2019 8,904 4 View

Which biochemical modification of Hemoglobin can potentially carry more oxygen to bloodstream?

Any synthetic analogue of Hemoglobin or any other Xenobiological agents can be cited as example. Please provide links and references.

10 November 2019 2,970 4 View