What is the maximum time for ligation mix incubation at 16C?

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José Alberto Núñez-Díaz

I prefer not to freeze the ligation mix at -20ºC. Freezing could damage plasmid DNA for future transformations. Normally I use all ligation mix in several transformations. I've used ligation mix stored for 5 days at 4ºC.

Hope this helps

Jasmin Šutković

Thanks, I worked out...after one day of -20 storage the ligation mix worked well. The transformation resulted in significant number of colonies on Agar plate...