Hello everyone,
I am trying to assign ~500 fluorinated compounds to different cocktails/samples so that there is no overlap even their chemical shifts change significantly (e.g. 30 Hz, or almost 0.1 ppm for a 400MHz spectrometer) when binding to targets.
Now I wonder, practically, how many compounds can be put in a cocktail? If I only try to avoid peak overlap, I could easily do 12 or more in a cocktail. Yet, I have only seen publications putting 8 or 10 compounds in a cocktail, and I am curious why?
Thanks for any suggestion.
Regards,
Yun
Yun
the typical number for 19F cocktails is 30. This requires smart selection to avoid overlap. Depending on the experiment you are using for screening you need to consider bandwidth. If you use a CPMG sequence with 180 degree pulses you will have to keep aromatic CF together and CF3's together. Otherwise the signals will be spread too far.
Rather than shift you will be looking for line broadening.
Clemens
Hi Clemens,
Did you mean 30 compounds/fragments per cocktail/sample?
I also found that line broadening is hard to observe (only 5uM target/protein) and sometimes the automatic shimming is not consistent among samples to the point that the peaks are broader in the control than with the target protein present.
Thanks,
Yun
Yun yes 30 fragments per sample. I am a bit surprised. The line should get quite a bit broader. What experiment are you using?
Clemens
- Badges
- Science method