What is the mathematical relationship between degree of extraction and CO2/sample mass ratio in SFE?

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Is it legally permissible to reuse algae biomass following the treatment of non-hazardous laboratory waste?

Hello, community, Could you please clarify whether current legislation permits the reuse of algae biomass after it has been used to treat non-hazardous decontaminated laboratory organic waste?...

05 June 2024 5,040 1 View

Metai ion (zinc2+) docking score calculation?

Is there a way to calculate the docking score of the zinc bound to this amyloid protein https://www.rcsb.org/structure/1ze9 without undergoing the docking procedure which is apparently not...

01 May 2024 8,951 1 View

Are there any open-source software for 1D/3D-2D coupling models?

Hello everyone, I was wondering if there is any available software that allows to do multiscale coupling. In particular, I'm working on 1D blood flow in microvascular networks embedded in 3D or 2D...

16 April 2024 7,124 0 View

Multinomial logistic regression with one independent variable?

Hi everybody! :) I wish to investigate a possible prediction between two variables I'm currently studying, and wanted to use a logistic regression model. Is it possible to run a multinomial...

07 August 2023 4,292 8 View

How to verify that you did an orthotopic injection into mammary fat pad and not a subcutaneous one?

Hi, I am trying to prepare for an orthotopic injection with breast cancer cells (D2.A1 and D2.OR). And I have a question is there any way to visualize the difference between a subcutaneous...

21 March 2023 9,634 0 View

What are the best contact points on our skin to record an ECG? Have new areas been discovered?

As technology advances, cables and classic electrodes will disappear, AI and sensors will take hold instead. New monitoring devices will have to be developed, they will be less invasive, smaller,...

12 January 2023 7,969 3 View

Are there any applications of AI in Electrocardiogram (ECG)?

AI to recognize cardiac arrhythmias like by using thermoscanner and other physiological sensors. New way and new product without the classic electrodes.

10 January 2023 5,442 5 View

Bleed through 680-800 IRDye LiCor?

Dear all, I am wondering if you have ever experienced bleed-through using the 680and 800 infrared dye secondary antibodies from LiCor. I observed a possible bleed through from a highly expressed...

05 September 2022 1,607 0 View

How to purify a hydrophobic protein with His-Tag?

I am trying to purify an hydrophobic recombinant protein containing a His-Tag. Is there any suggestion about lysis and wash buffers? To eluate I am using imidazole.

06 April 2022 5,748 4 View

Method to generate keywords out of a scientific text?

Which method of text analysis should I use if I need to get a number of multiword keywords, say (up to) 5 per text, analysing a text of some length? In particular, the text could be a) a title, b)...

28 March 2022 4,001 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Ethylene glycol is newtonian or non newtonian fluid?

Some sources say it is treated as Newtonian as ethylene glycol has a higher viscosity than water, which affects the flow characteristics in simulations. But some also say it is non-Newtonian.

09 August 2024 2,111 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View