Is it in the range of micro molars?
Are there any references that I an look at?
Mrs/Miss Fathollahipour,
LOD = 0.3 mg L-1, 470.59 MHz instrument (African J. Pharmacy and Pharmacology 5, 1573-1579, 2011).
There is no simple answer to this question. It will depend on the probe in addition to the magnetic field strength. With a cryogenically cooled probe the LOD will be quite a bit lower than .3 mg/l. It will also depend on how long you will data acquisition to go on or how large the molecule is. I would estimate that under good conditions you would be able to detect less than one nano Mol of material. Do you just need to identify a 19F signal or do you need to quantify? Identification would be dependent on LOD whereas quantification would be LOQ which would be require more material or longer data collection.
Clemens
Dr. Ivanova,
Thanks a lot for your helpful reply. Are there any literature that I can use as a reference for what you said?
I'd appreciate it
Dr. Anklin,
Thanks a lot for your valuable answer. Can you please let me know if there are any references so I can use as a reference for this statement?
Thanks
Mrs/Miss Fathollahipour,
The reference is already shown as bibliography in the parentheses.
You should bear in mind, however, that important is the so-called limit of quantitation (or limit of quantification) (Both terms are equally distributed in the literature) which represents the down confidence limit of the calibration curve, rather than LOD.
LOQ = (3.3).LOD
This means that in this case LOQ = 0.99 mg.L-1 (at the end of the linear calibration range). Because of LOD is at the end of the dynamic range, but it is non-linear and could broadly vary. For this reason you could observe signal, but its reliability in quantitative terms is low. Or you operate with great uncertainty:
- LOD < C > LOQ the analyte can be detected but not quantified.
- C > LOQ – meaningful quantitative information.
You could pay attention to ISO 11929/2010 for more detail about the 'decision threshold' and ISO 98-3/2008 towards 'uncertainty in the measurements' (https://www.iso.org/standard/43810.html).
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