What is the link between Arab criticism and Western criticism ?

More د.حوراء ابراهيم جاسم الموسوي's questions See All

Is there a statistical solution to the problem of weak responses?

Is there a statistical solution to the problem of weak responses to a scientific research questionnaire that gives an indication of results to a certain extent that are reliable and can be...

08 January 2021 8,453 2 View

In your opinion, as an educator which is better for the child, traditional play or electronic play, and why?

In your opinion, as an educator, researcher, or guide, which is better for children in our time, traditional or electronic play, and why

01 January 1970 9,065 18 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

What is the effect of SDS running buffer concentration on western results?

I am trying to do a thermal shift assay for a recombinantly-produced membrane protein, by western blot band quantification. I use Bio-rad 10X Tris/glycine SDS running buffer for my SDS-PAGE and...

11 January 2021 5,302 2 View

How to quantify total protein in crooked western lanes?

I've been using Revert700 total protein stain to detect total protein in my western lanes. While this stain is robust & reversible, I have trouble quantifying in Image Studio Lite (LiCor) and...

05 December 2020 9,014 3 View

Why does immunofluorescence but not western blot works for fusion protein?

I have a flag-tagged protein and want to be sure it is properly expressing in my cells. When I assess by IF, I see clear nuclear enrichment (expected). But when I do a western, there is only a...

27 November 2020 2,350 3 View

Could you kindly assist with the journals that are related to my title below?

Effects of covid-19 pandemic on micro companies within the event sector in Western Cape.

08 November 2020 2,138 2 View

How to increase the signal of the protein standard when performing western blot?

Hi, I'm writing my master's thesis on the characterization of human proteins which I express and purify from E. coli. I'm performing western blot to verify my protein, but the protein standard...

06 October 2020 1,107 5 View

Why am I getting multiple bands in western blot (in Isolating adhesion complexes) ?

Hello everyone, I am isolating adhesion complexes and wanted to confirm the presence of a specific protein in the isolated complex. When I performed western bott, I got a band at the right size...

19 September 2020 538 7 View

How I explain the increase both of the p-AKT and AKT in the western blot?

Hi, I would like to ask a question, the cell treated with drug for 48h, and the cell viability was changed using cck-8 kit, so I think the p-akt is decreased in western, however, the p-akt was...

07 September 2020 4,880 4 View

Can someone point me towards a commercially available E1b19K antibody against Human Ad5-E1b-19K?

#antibody #western #Ad5 #E1b19K

16 August 2020 9,283 1 View

Anti-His Antibody binding everything on a western?

Hello all, I've been expressing some His-tagged proteins in E.coli that appears to be going just fine based on how the SDS-PAGE gels look, so I wanted to confirm with a Western. I transferred my...

22 July 2020 5,255 5 View