On a substrate, the human periodontal ligament fibroblasts reached confluence on day one because of high cell seeding density. Therefore, i am interested to know how long can these cells survive while being in this confluent state?
It is a known fact that primary cells have finite lifespan. I am also working with human primary cells but only up to 3 passages.
I have trained the vision transformer several times and noticed that it takes so much time to train the network while working with images, especially when we increase the number of transformer...
28 October 2022 1,607 2 View
Hello friends, I encountered a problem regarding the evaluation of semantic segmentation. Qualitatively the ground truth and prediction are quite similar but dice shows a small number about 0.56,...
24 October 2022 4,112 3 View
I have to do proximal mapping of a complex vector p onto the surface of M dimensional complex sphere, if someone can suggest to me some literature and toolboxes from which I can get some basic...
04 September 2022 8,544 0 View
In order to design a reconfigurable unit cell at 28 GHz using a varactor diode, I searched the data sheet to know the values of the lumped elements, i.e., R and L. However, the data sheet is...
29 June 2022 1,774 1 View
and for Total Variation optimization how to frame the objective function having L1 norm function then what is the alternative way of imposing Total variational regularizer .
17 February 2022 5,224 4 View
HEK293F cells grow in suspension culture and need constant shaking for growth that requires a CO2 incubator equipped with shaker. Has anyone tried alternative ways to culture these cells when...
08 January 2022 6,513 4 View
Hi, Will the exchange current density be constant for the case where we use the power of a capacitor for an alkaline electrolyzer to produce hydrogen?, Or should it change when the capacitor...
12 August 2021 1,338 3 View
I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.
03 March 2021 5,885 4 View
Hello everyone, I need to annotate the MRI images (with .nii.gz format). Each image includes several slices. What I need is an annotation tool that can annotate the area of interest and propagate...
28 February 2021 3,557 9 View
I am looking for Dataset regarding PARM.. someone can share links or Email me on [email protected]
27 November 2020 1,012 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...
07 August 2024 8,414 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View