What is the largest molecule or complex that can go through the nuclear pore?

Gabriel Monteiro da Silva @Gabriel-Monteiro-Da-Silva

21 October 2019 1 4K Report

I'm trying to figure out if a complex formed by one of the proteins I'm studying not too large to be imported to the nucleus, but I'm having trouble finding data about the boundaries of what is thought to be the maximum size that a protein/complex can be to pass through the pore.

Also, do you know how long (in nM) would a 6kb RNA chain be?

Pierre-Emmanuel Gleizes

The nuclear pore complex can accommodate very large cargoes, for example:

- Ribosomal subunits (20-25 nm, 1-3 MDa) [Delavoie et al., Nature Com. 2019]Article The path of pre-ribosomes through the nuclear pore complex r...

- Gold particles up to 39 nm in diameter [Panté and Kann, Mol. Cell. Biol. 2002]Article Nuclear Pore Complex Is Able to Transport Macromolecules wit...

- Viruses [Fay and Pante, Frontiers in Microbiology, 2015; doi: 10.3389/fmicb.2015.00467 ]

- the very large Balbiani-ring mRNPs [Stevens and Swift, J Cell Biol 1966; DOI: 10.1083/jcb.31.1.55 ]. In that case, the structure of the mRNP changes to accommodate the size of the central channel of the NPC.

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