Software for bond manipulation with POSCAR (VASP) files?

I am calculating adsorption energies of molecules on surfaces using VASP. Every time I want to modify the starting configuration of the molecule, I am doing this long process of converting...

19 January 2021 2,624 3 View

Does anyone know of an anti-rat CLEC-2 antibody for IHC?

I am looking for an anti-rat CLEC-2 antibody for IHC in rat lungs. Has anyone come across one? There are a few mouse ones but the data sheets don't say they cross react with rat, and the ones that...

06 December 2020 3,511 0 View

What are the efficient methods available to test the soil fertility?

Want to understand the scientific mechanisms to know type of soil suitable for agricultural purpose.

22 October 2020 4,941 3 View

NirS qPCR assay underestimating by 2 log?

My lab designed a qPCR assay for amplifying the denitrification gene nirS from DNA extracted from soil. The assay has worked perfectly in the past with almost perfect efficiency and gene copy...

20 July 2020 8,070 1 View

Why does Gaussian opt+freq job optimizes fine but crashes during Frec calc?

I am running jobs like # opt freq uwb97xd/6-311g(d,p) integral=grid=ultrafine geom=connectivity They complete the optimizations steps and then die during force calculations. An example of the...

08 July 2020 7,456 1 View

How should I respond to this warning message in Gaussian 09?

When running jobs similar to: # opt freq ub3lyp/6-311g geom=connectivity I often get this warning message: **** Warning!!: The largest alpha MO coefficient is 0.20327268D+02 Is this a...

13 May 2020 4,122 7 View

Does anyone know of any publications related to compulsory research as part of postgraduate specialist medical training?

I am trying to assess the impact of the introduction of a compulsory research study as part of a 4 year postgraduate medical specialist training (residency/registrar/ coursework Master's) in South...

13 January 2020 781 3 View

How deep should be deep learning? How to decide the depth?

Deep learning is one of the hot topics, the entire world is talking about. So many advantages are there with deep learning, along with its own limitations. More deep we make the design, the time...

30 December 2019 4,744 4 View

What's the best way to extract DNA from buccal cells (taking into account yield, quality, time and cost)?

I'm looking into extracting DNA from buccal cells. I have no experience in using this source material and was wondering if there was a general consensus on preferred method? Yield would be a...

27 November 2019 240 3 View

Can I use standard plastic falcon tubes for chloroform DNA extraction?

Are there any significant issues with using standard plastic falcon tubes for DNA extraction with chloroform? How long can chloroform be in contact with polypropylene for it to have a negative...

17 October 2019 3,713 4 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

Is there anyone who is good with DNA extraction from hops?

Hi I extracted the DNA using the CTAB method and purified it with a kit, then I get this band like in the picture below. I am desperate, what can I do!

16 February 2021 5,508 3 View

How to calculate the sample size required to detect the difference in pharmacokinetics of methotrexate?

I came across with this study here, https://www.clinexprheumatol.org/abstract.asp?a=5066 And this study says: "The sample size calculation was done to compare two different MTX starting dose...

14 February 2021 3,017 3 View

How to calculate ultraviolet dose in Gy or J/kg?

Currently I'm doing a literature review regarding the comparisons of different types of radiation such as uv, electron beam and gamma radiation. But I'm having a difficult time converting uv unit...

11 February 2021 7,642 3 View

What is the maximum wavelength and absorbance for dodecyl trimethylammonium bromide?

I am measuring the absorption of dodecyl trimethylammonium bromide surfactant using UV spectrometer. I am trying to set the wave length range for the experiment. Please does anyone have a idea of...

07 February 2021 7,720 1 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

How to improve my BrdU immunostaining?

24 January 2021 414 3 View

How to improve the yield for the reaction below?

I am trying to increase the yield for the reaction below. I did the column and I saw that the major side-product is the symmetric dimer formed by 4-bromobenzyl bromide. I imagine that NaH is...

20 January 2021 7,874 4 View