While working on the effects of varying temperatures on the embryonation and development of Culex pipiens, I noticed mosquitoes lived longer though smaller in size, yet developed fast in higher temperatures. Looking for explanations, I read something on Shock proteins having a role. Can I please get more information on this?
Dear Martha;
An organism's response to environmental and physiological stressors includes a highly ordered set of events that are often represented by rapid changes in heat shock proteins (HSPs) gene expression followed by synthesis of the proteins involved in adaptation (Staib et al., 2007). HSPs are a group of highly conserved cellular proteins synthesized in response to a variety of stressors (Sanders, 1990, 1993). They are classified, according to their molecular weight, into HSP90, HSP70, HSP60, and smaller HSP families which are present in all organisms including fish (Basu et al., 2002). As some studies investigated that the most sensitive to heat shock is HSP70. Therefore, you may use HSP60, HSP70 and HSP90.
Regarding the primer sequences, I suggest you to check it in NCBI:
Primer design and check up for related gene sequences: http://blast.ncbi.nlm.nih.gov/Blast.cgi
Primer designing tool: http://www.ncbi.nlm.nih.gov/tools/primer-blast/
Best regards
Michael
nY nterest in your work had to do with the temperature of the site where the mosquitoes stings the person. But I have no idea of your specific problems. My area of research is within the role of the Arteriovenous anastomoses (the AVAs) on the temperature distribution in the forearms, as they feed the subcutaneous venous retes in the forearms and arms. I am sorry that I cannot supply any relevant informations to your research, Leif Vanggaard.
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