How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Why are readings on an Eppendorf BioFlo 120 bacterial bioreactor lost after a few hours?

Hello all, Our lab has a bacterial bioreactor from Eppendorf (BioFlo 120) that is normally set to read/record trend data (pH, temperature, etc.) over a set number of hours (overnight). We...

26 January 2021 8,192 1 View

How to de-clump HEK293 suspension cells?

I have a problem with clumping of HEK293 suspension cells, and this clumping problem decreases the cell viability. I will transfect the cells if I were to reach a favorable cell count. What can I...

17 January 2021 354 4 View

Why there is no increase in virus titer after 24 hour of seeding for harvest?

I am working on virus production through bioreactor,my concern is regarding ,no increase in virus titer after 24 hour of seeding for harvest Usually we seed the fermenter and incubate for 72...

05 January 2021 3,860 2 View

Best Way to Preserve Nitrifying Bacteria in Liquid Culture?

I have a heterogeneous culture of nitrifying bacteria that was originally isolated from the field. It has been kept alive for several months in a bioreactor. I want to pull some of this...

18 December 2020 5,411 4 View

How Can I convert OD550 (Optical Density at 550 nm) to NTU (Nephelometric Turbidity Unit)?

OD and NTU are two different units, that can be measured through a spectrophotometer and Bioreactor (integrated with turbidity sensor) respectively. Because of the difference in units, the results...

03 October 2020 5,662 2 View

How to draw a operating window to demonstrate the bioreactor performance?

I would like to make a operating window for a bioreactor process where I want to incorporate data such as impeller speed, gas flow rate, hydrodynamic shear, mass transfer efficiency, power...

01 September 2020 2,501 3 View

Hi, i want to scale up my recombinant protein production from flask to 5L fermentor can i use my optimized condition in the flask for fermetor?

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12 July 2020 5,340 3 View

Can anybody suggest how to calculate the volume of a fed-batch bioreactor?

I've done mass balance around the reactor but I'm stuck at the volume bit because volume is not constant.

28 March 2020 8,702 3 View

Can trial reactors be setup without replicate (duplicate) in semi-continuous long term AD experiments?

I am planning a new set of AD experiments (agricultural waste as a substrate), and if I run a duplicate of my trials, I will have a total of 6 reactors (this seems a lot of work since I have to...

18 February 2020 6,904 5 View

Can trial reactors be setup without replicate (duplicate) in semi-continuous long term AD experiments?

I am planning a new set of AD experiments (agricultural waste as a substrate), and if I run a duplicate of my trials, I will have a total of 6 reactors (this seems a lot of work since I have to...

01 February 2020 10,072 4 View