Anyone know how to measure catalase activity in brain homogenates in terms of μmol/min/g of brain. Reaction was stopped by addition of dichromate and absorbance was measures after 1 min at 570 nm. Controls are without H2O2 for each samples.
Shail K Chaube
There are several tests available to measure the catalase activity in brain homogenates. Hope you are trying to analyze enzyme activity using colorimetric assay kit. We have used the sensitive kit purchased from BioVision, Inc., CA that is very sensitive reproducible. If you are using the colorimetric assay kit, follow our paper published in Brain Research Bulletin 95 (2013) 54– 60. For more detail, see our research paper. Follow the kit mannual for calculating the catalase activity. All the best.
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