Which anesthetic for cortisol determination in fish?

I am a bit confused. I am planning an experiment in the field where fish will be caught by electrofishing. Afterwards, they are supposed to be euthanised by using an anaesthetic. I found...

10 February 2021 1,101 4 View

Can peptides survive high temperatures?

I'm looking for ways to functionalise a material with a cell-adhesive peptide (relatively short chain - 6aa) and I was just wondering if peptides can withstand high temperatures (100-150 C) for 30...

15 January 2020 8,978 4 View

Abnormally high fluorescence read with qpcr, and no amplification showing on agarose gel, what could be going on?

Hi! I've been running qPCR on some drinking water samples with a primer/ probe FAM assay. In any given run, there have been at least a few samples with artificially high fluorescence reads,...

16 October 2019 8,060 3 View

How to prepare agar amended with mercuric chloride?

Several studies mention using agar amended with mercuric chloride when isolating mercury resistant bacteria. Is there a lab method available for preparation of these plates? If no, what are some...

17 June 2019 5,047 5 View

Interview guides for UTAUT?

30 January 2019 5,443 4 View

How much does our childhood affect the way we think and make investment choices?

Subconscious, subjective drivers.

22 January 2019 5,397 5 View

ReNcell VM cell culture problems - why are they dying after I split them?

The cells look healthy after thawing, but they die when I try to split them. I have tried making fresh media and fresh matrigel, and have tried filtered and non-filtered Accutase. Does anyone have...

19 December 2018 630 9 View

Is it necessary to dechorionate zebrafish embryos to extract genomic DNA and RNA ?

I tried to dechorionate embryos with pronase at 1mg/mL. I made 3 washes but I obtained a high mortality rate and the medium was dirty. Thank you for your answers

03 January 2017 3,455 4 View

Can you help me with Activity measure of trypsin?

Sustrate=BAPNA I get a white solution when i mixed BAPNA(with DMSO) and buffer Tris-HCL/CaCl2 First 3 minutes the activity goes down, after 3 min stars to go up but slowly. Is this normal?

25 July 2016 6,718 7 View

How do I transfer my data from Spss version 22 or excel into WHO anthroplus and how do I categorize ages which are in decimals in spss?

My data has been coded 1=male and 2=female, I got a response that my variables were invalid when I imported into WHO Anthroplus from my excel worksheet. My aim is to calculate the BMI-Z=Scores of...

02 November 2014 7,355 17 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View