I made ethanol extract of a plant material and now I want to dissolve it in a suitable solvent to study its anticancer effect and also to detect and determine its active ingredients.
hello,
The best way to test the activity of your ethanol extract is not to use solvents.
For that you lyophilized extract or dissolved it in water after evaporation of ethanol.
Dear Thoria
After the solvent was evaporated you can use 10% dimethyl sulfoxide (DMSO) to reconstitude the extract and make serial dilutions in a suitable cell culture medium. See the method of Morsmann, T 1983 J Immunol methods 65 p263-271
regards
Dear Thoria
From mine opinion you must check the soulbility of your extract in water and 10 % DMSO. You may enahnce the solubility of your extract by sonicating the solution.
Usually ethanol extract are not soluble in water. The extract has to be dissolved in 10% DMSO. This enhances the solubility of the extract. You can now prepare serial dilutions of the extract before application.
ethyl acetate best solvent to give tocopherol inside another antioxidants
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